Figure 5.

Comparison of Effects of Mutations on Peptide Binding in Competition ELISAs and Phage-DMS

(A and B) Bar plot showing the IC50 values of wild-type and mutant peptides in a competition ELISA for (A) gp41-specific mAbs with wells coated with MN gp41 protein or (B) V3-specific mAbs with wells coated with SF162 gp120 protein. Antibodies were pre-incubated with each peptide before addition to the wells. Peptides for which no inhibition of antibody binding could be detected at any concentration tested are indicated with an infinity symbol. Results for three replicate experiments are shown, with the mean ± SEM. Statistical significance was determined by an unpaired t test.

(C and D) Correlation between results of IC50 values from competition peptide ELISAs and scaled differential selection values from Phage-DMS for (C) gp41-specific mAbs and (D) V3-specific mAbs. Pearson's correlation shown at the top.