REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Oral Polio Vaccine Sabin Type 2 reference strain (OPV2)	NIBSC	01/530
Poliovirus Type 3 Leon	NIBSC	PV3 Leon
Biological Samples
Immunized mouse sera	This study	N/A
Human serum samples	Van Damme et al., 2019	N/A
Human feces samples	Van Damme et al., 2019	N/A
Chemicals, Peptides, and Recombinant Proteins
Bovine albumin fraction V, 7.5%	GIBCO	15260037
Formaldehyde solution	Sigma	252549
Crystal violet	Sigma	C0775
Methanol	Fisher Scientific	A412-500
Critical Commercial Assays
KAPA Stranded mRNA-Seq Kit with KAPA mRNA Capture Beads	KAPA Biosystems	Cat# KK8420
HiSeq Rapid Cluster Kit v2	Illumina	Cat# GD-402-4002
HiSeq Rapid SBS Kit v2	Illumina	Cat# FC-402-4022
Miseq Reagent Kit v2 (300 cycle)	Illumina	Cat# MS-102-2002
ZR Viral RNA Kit	ZYMO Research	Cat# R1035
SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity DNA Polymerase	Invitrogen	Cat# 12574030
AMPure XP magnetic beads	Beckman Coulter	Cat# A63880
Qubit High Sensitivity dsDNA assay	Life Technologies	Cat# Q32854
Luciferase assay system	Promega	Cat# E1500
Phusion High-Fidelity DNA Polymerase	NEB	M0530S
Deposited Data
In vitro accelerated evolution under higher temperature	This paper	SRA: PRJNA605034
Poliovirus shed from OPV vaccinees	This paper	SRA: PRJNA605034
Variant calls for deep sequencing	This paper	Mendeley data https://doi.org/10.17632/3n5ztwx66m.1
Experimental Models: Cell Lines
Human: HeLa S3 cells	ATCC	CCL2.2
Human: Hep 2C cells	NIBSC	740502
Monkey: Vero cells	ATCC	CCL81
Mouse: L20B cells	NIBSC	081102
Experimental Models: Organisms/Strains
Mouse: PVRTg21-IFNR-ko	Gift from Satoshi Koike, Tokyo Metropolitan Institute of Medical Science	Tg21/IFNR-ko
Mouse: PVRTg66	NIBSC	PVRTg66
Oligonucleotides
NEXTFlex RNA-Seq Barcodes	BIOO Scientific	#512914
Primer D53N-F (5′-GCTCAGGACAAATTTTG AAGAAGCAATATTCTC-3′)	This paper	D53N-F
Primer D53N-R (5′-CTTCAAA ATTTGTCCTGAGCCTGGGAT CATTTTTG-3′)	This paper	D53N-R
Primer K38RF (5′- GAAGGGGTGAGAGA ACCAGCAGTCCTTACCAA-3′)	This paper	K38R-F
Primer K38RR (5′- CTGCTGGTTCTCTCAC CCCTTCAAACACATAGTG-3′)	This paper	K38R-R
Primer P234SF (5′- CATCACTCAGCTCAGCC TGGTTTGAGGCACTCAA-3′)	This paper	P243S-F
Primer P234RR (5′- CAAACCAGGCTGAGCT GAGTGATGCATCATAACC-3′)	This paper	P243S-R
Primer L445MF (5′- GGGAGAGCTGTGTTGCT CCCAGAGTACTCTACATTGTAC-3′)	This paper	L445M-F
Primer L445MR (5′- CTGGGAGCAACACAG CTCTCCCAATTGGCACACTCCTG-3′)	This paper	L445M-R
Primer S2-d3D-F (5′- GGATGAAAC CATGATAATAAGTGGGATACCCG ATCATAAATGCTCC-3′)	This paper	S2-d3D-F
Primer S2-d3D-R (5′- GGGTATCC CACTTATTATCATGGTTTCATCC ATTGAATCTCACCTTG-3′)	This paper	S2-d3D-R
Primer F-S2REP-5UTR-EcoRI (5′- CAT GATTACGAATTCGAGCTCTAATAC-3′)	This paper	F-S2REP-5UTR-EcoRI
Primer R-S2REP-5UTR-SalI (5′- CTTCC ATGTCGACAACTTTCTGTGATGAA ACTTGGGCGC-3′)	This paper	R-S2REP-5UTR-SalI
Primer F-S2REP-FLuc-SalI (5′- GAAA GTTGTCGACATGGAAGACGCCA AAAACATAAAG-3′)	This paper	F-S2REP-FLuc-SalI
Primer R-S2REP-FLuc-EagI (5′- CTAGT GGCGGCCGCACGGCGATCTTTCCG CCCTTCTTGG-3′)	This paper	R-S2REP-FLuc-EagI
Primer F-S2REP-NSR-EagI (5′- GCCG TGCGGCCGCCACTAGAAAAG GGATTAACGAC-3′)	This paper	F-S2REP-NSR-EagI
Primer R-S2REP-NSR-SbfI (5′- GGTAAC GCCAGGGTTTTCCCAGTCACGACG-3′)	This paper	R-S2REP-NSR-SbfI
Recombinant DNA
Plasmid: pRA-Sabin2	This paper	pRA-Sabin2
Plasmid: pRA-Sabin2-D53N	This paper	pRA-Sabin2-D53N
Plasmid: pRA-Sabin2-K38R	This paper	pRA-Sabin2-K38R
Plasmid: pRA-Sabin2-D53N/K38R	This paper	pRA-Sabin2-53/38
Plasmid: pS2/S15domV/cre5	This paper	pRA-Sabin2-2909
Plasmid: pS2/S15domV/cre5-D53N	This paper	pRA-Sabin2-398/481/2909
Plasmid: pS2/S15domV/cre5-K38R	This paper	pS2/S15domV/cre5-K38R
Plasmid: pS2/S15domV/cre5-D53N/K38R	This paper	pS2/S15domV/cre5-D53N/K38R
Plasmid: pSabin2-d3D	This paper	pSabin2-d3D
Plasmid: pSabin2-dCre	This paper	pSabin2-dCre
Plasmid: Sabin2 replicon	This paper	pS2Rep
Plasmid: pS2Rep-D53N	This paper	pS2Rep-D53N
Plasmid: pS2Rep-K38R	This paper	pS2Rep-K38R
Software and Algorithms
Prism	GraphPad Software	https://www.graphpad.com
LoFreq	Wilm et al., 2012	https://csb5.github.io/lofreq/
Python	Python Software Foundation	https://www.python.org
Chimera	UCSF	https://www.cgl.ucsf.edu/chimera/