Table-1.
Primers used to evaluate the presence of resistance genes [42] in Salmonella spp. strains.
| Antibiotic | Target gene | Primer sequence | Amplicon size (bp) |
|---|---|---|---|
| Ampicillin | blaPSE-1 | F- GCAAGTAGGGCAGGCAATCA R- GAGCTAGATAGATGCTCACAA |
461 |
| blaTEM | F- ATCAGTTGGGTGCACGAGTG R- ACGCTCACCGGCTCCAGA |
608 | |
| Chloramphenicol | catA | F- CCAGACCGTTCAGCTGGATA R- CATCAGCACCTTGTCGCCT |
454 |
| cmlA | F- TGGACCGCTATCGGACCG R- CGCAAGACACTTGGGCTGC |
642 | |
| Florfenicol | floR | F- CACGTTGAGCCTCTATATGG R- ATGCAGAAGTAGAACGCGAC |
888 |
| Gentamicin | aadB | F-CTAGCTGCGGCAGATGAGC R-CTCAGCCGCCTCTGGGCA |
300 |
| Streptomycin | aadA1 | F- CTCCGCAGTGGATGGCGG R- GATCTGCGCGCGAGGCCA |
629 |
| aadA2 | F- CATTGAGCGCCATCTGGAAT R- ACATTTCGCTCATCGCCGGC |
501 | |
| strA | F- TGGCAGGAGGAACAGGAGG R- AGGTCGATCAGACCCGTGC |
404 | |
| strB | F- GCGGACACCTTTTCCAGCCT R- TCCGCCATCTGTGCAATGCG |
620 | |
| Trimethoprim | dfrA1 | F- CAATGGCTGTTGGTTGGAC R- CCGGCTCGATGTCTATTGT |
253 |
| dfrA10 | F- TCAAGGCAAATTACCTTGGC R- ATCTATTGGATCACCTACCC |
433 | |
| dfrA12 | F- TTCGCAGACTCACTGAGGG R- CGGTTGAGACAAGCTCGAAT |
330 | |
| Ceftriaxone | blaCMY2 | F- AAATCGTTATGCTGCGCTCT R- CCGATCCTAGCTCAAACAGC |
244 |
| blaCTX-M | F- TTCGCTAAATACCGCCATTC R- TATCGTTGGTTGTGCCGTAA |
236 | |
| Sulfamethoxazole | sul1 | F- CGGACGCGAGGCCTGTATC R- GGGTGCGGACGTAGTCAGC |
591 |
| sul2 | F- GCGCAGGCGCGTAAGCTGAT R- CGAAGCGCAGCCGCAATTC |
514 | |
| sul3 | F- GGGAGCCGCTTCCAGTAAT R- TCCGTGACACTGCAATCATTA |
500 |