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. 2020 Oct 2;11:564699. doi: 10.3389/fimmu.2020.564699

Figure 2.

Figure 2

Lipopeptides mimicking bacterial lipoproteins induce higher BAFF expression compared with other microbial products in DCs. (A) DCs were stimulated with 0-10 μg/ml of Pam3CSK4, Pam2CSK4, standard LPS (stdLPS), or ultra-pure LPS (upLPS) for 48 h. (B) DCs were stimulated with Pam3CSK4 (10 μg/ml), poly(I:C) (25 μg/ml), peptidoglycan (10 μg/ml), or flagellin (100 ng/ml) for 48 h. (C) DCs were stimulated with 0–10 μg/ml of Pam3CSK4, LTA from S. aureus, or zymosan for 48 h. After stimulation, the cells were stained with CD11c antibodies conjugated with APC and BAFF antibodies conjugated with FITC, and subjected to flow cytometry. The histograms are representative results from flow cytometry for mBAFF from each treatment group, and the value given in each histogram is the percentage of mBAFF-positive cells among 10,000 events of CD11c-positive cells.