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. 2020 Oct 2;11:564699. doi: 10.3389/fimmu.2020.564699

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Copyright © 2020 Im, Baik, Lee, Park, Park, Yun and Han

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pam3CSK4-induced BAFF expression is mediated through TLR2 and MyD88. (A) DCs from wild-type, TLR2- or MyD88-deficient mice were stimulated with Pam3CSK4 at 0-10 μg/ml for 72 h. The cells were then stained with antibodies for mouse CD11c conjugated with APC and BAFF conjugated with FITC, and subjected to flow cytometry. The histograms are representative results from flow cytometry for mBAFF, and the value in each histogram is the percentage of mBAFF-positive cells among 10,000 events of CD11c-positive cells. (B) Under the same culture condition, sBAFF levels in culture media were measured by ELISA. The values are the mean ± standard deviation of three separate experiments. Asterisk (*) indicates a significant difference at p < 0.05 between the non-treatment and treatment groups.