FIG 1.

Sanger sequencing results and alignment to SARS-CoV-2 S gene. Sequence was aligned to SARS-CoV-2 S gene (GenBank MT577641.1) using NCBI BLASTN. Highlighted gray, forward and reverse primers; highlighted yellow, aligned sequence; highlighted purple, mismatched sequence from either primer. The first 24 bases in the COVID-19 S gene are the T7 promoter added by PCR with S-F and S-R primers in Table 1. Results for the Southern influent sample were similar. Sequencing chromatograms for forward and reverse primers are provided in Fig. S1B.