Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The binding free energy (kcal/mol) and binding constants of the WT-CA, F46A-CA, L65A-CA, and T94A-CA systems based on the fluorescence spectroscopy quenching method.
| WT-CA | F46A-CA | L65A-CA | T94A-CA | |
|---|---|---|---|---|
| ΔGbind (kcal/mol) | −12.16 | −0.38 | −2.86 | −1.77 |
| Binding constants K ml·µg−1 | 0.45644 | 0.12715 | 0.33597 | 0.32911 |
