Figure 5.

Upregulation ofperoxisome proliferator-activated receptor gamma (PPARγ) is responsible for lipid accumulation inengineered heart tissue (EHT).

A, Normalized PPARγ transcripts in wild-type and DNA methyltransferase (DNMT) 3A^−/− EHTs. n=3 EHTs per group, ***P<0.001. B, PPARγ promoter methylation in wild-type and DNMT3A^−/− EHTs measured by bisulfite sequencing. Black dot, methylated cytosine; white dot, unmethylated cytosine. C, Hematoxylin and eosin staining (H&E) staining of vehicle (left) and PPARγ-inhibitor GW9662–treated DNMT3A^−/− EHTs (right); scale bar, 50 µm. D, H&E staining of DNMT3A^−/− EHTs cultured for 4 weeks in serum-containing conditions; scale bar, 50 µm.