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. 2020 Sep 4;142(16):1562–1578. doi: 10.1161/CIRCULATIONAHA.119.044444

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© 2020 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 6. — Effect of DNA methyltransferase(DNMT) 3A knockout on engineered heart tissue(EHT) glucose metabolism.

Effect of restrictive feeding with lactate or glucose-only medium on frequency (A), force (B), and RR scatter, with R being the interval between 2 neighboring force peaks, (C) of wild-type and knockout EHTs. n=8, *P<0.05, ***P<0.001. D, Relative transcript abundance of glycolytic genes in DNMT3A^−/− #2 EHTs after 1 week of serum-free culture. n=3, values normalized to wild-type (dashed line). E and F, Quantification of hypoxia-inducible factor 1α (HIF-1α) protein by Western blot of DNMT3A^−/− #1 and isogenic control EHTs cultured either with lipoprotein-deficient serum (LDS), control serum, or dialyzed serum. Loading control = ERK1/2; n=2 to 3 EHTs per group.