Fig. 2.

(A) Heatmap analysis of RNA sequencing data from HGGs, revealing differential expression of candidate genes CCL2 and MMP9 in tumors from V/I + I/I group compared with V/V group. Two separate GSC lines, 6-27 (B) and 7-2 (C), with similar proneural characteristics but different CX3CR1 genotypes were immunostained with antibody against CX3CR1 (green) and counterstained with 4′,6′-diamidino-2-phenylindole (DAPI; blue), revealing robust membranous CX3CR1 expression. Stimulation of GSC6-27 (V/V) with CX3CL1 significantly increased CCL2 mRNA levels compared with treatment with PBS, whereas GSC7-2 (V/I) did not show this response (D; n = 3 for each condition). In addition, basal CCL2 mRNA levels were significantly lower in GSC7-2 compared with GSC6-27. *P < 0.05, unpaired t-test. Scale bar, 25 μm.