Figure 4.
Sildenafil treatment suppresses tumor growth in ovo. (A) 1.5 × 106 HCT-116 colon or MIA PaCa-2 pancreatic cancer cells were implanted 8 days after fertilization. Tumors were treated 24 and 48 h with 25 µM Sildenafil (Sil). Tumor photographs 96 h after implantation are presented. Scale bar, 1 mm. (B) Quantification of tumor area is presented. Error bars represent mean ± SEM of four to seven tumors. (C) IHC of HCT-116 and MIA PaCa-2 cells growing on CAM using specific antibodies directed against Ki67 are presented. (D) Quantification of Ki67 positive cells is shown. Error bars represent mean ± SEM of at least six microscopic fields, each containing at least 200 cells.