Figure 6.
Sildenafil potentiates PU-H71 effects toward impaired tumor growth. (A) Lysates of cancer cell lines incubated with different amounts of PU-H71 for 24 h days were subjected to western blot analysis with PARP-specific antibody. β-Actin was used as loading control. (B) 1 × 106 SW480 colon cancer cells were implanted inside a silicon ring on chicken CAM 8 days after fertilization. Tumors were treated either with 50 nM PU-H71, 15 µM Sildenafil or combination of both compounds at 24 and 48 h after implantation. Tumors were allowed to growth for additional 24 h. Scale bar, 1 mm. (C) Quantification of tumor area is presented. Error bars represent mean ± SEM of five to seven tumors. (D) IHC of SW480 cancer cells growing on CAM using specific antibodies directed against Ki67 is presented.