Figure 2.

Correlation of autopsy histopathological findings with POWV detection in Subjects B and C. A, Autopsy tissue collected from Subject B included lung (a), heart (b), liver (c), spleen (d), prostate (e), kidney (f), right testicle (g), left testicle (h), and bone marrow (i). Foci of chronic inflammation were identified in the left testicle, while the remaining specimens showed minimal, nonspecific abnormalities without histological evidence of infection or recurrent lymphoma. B, Brain sections from Subject B included basal ganglia (a), frontal cortex (b), periventricular white matter (c), midbrain (d), thalamus (e), pons (f), and cerebellum (g). Findings of meningoencephalitis, including lymphocytic predominant leptomeningeal, perivascular, and parenchymal inflammation, microgliosis with microglial nodules and neuronophagia, and severe neuronal loss, were present throughout the brain and most predominant in the cerebellum. C, Brain sections from Subject C included frontal cortex (a), thalamus (b), medulla (c), and cerebellum (d). Histological abnormalities were similar to Subject B and most severe in the brainstem and cerebellum. All histological images are of hematoxylin and eosin–stained slides using a 20× objective. The quantity of virus detected by metagenomic sequencing (POWV reads/million) is indicated by the thickness of the boxes surrounding the histological images. Anatomy figures were obtained from Wikimedia Commons under Creative Commons CC0 1.0 Public Domain Dedication. Abbreviation: POWV, Powassan virus.