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. 2020 Oct 15;10:17446. doi: 10.1038/s41598-020-74296-2

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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APP-A4 protein expression following Double Blast Exposure. (a) Histograms representing the densitometric ratio of levels of APP-A4 with respect to GAPDH as measured in the frontal cortex, hippocampus, cerebellum and brainstem in the brains of rats 15-days after an explosive-driven double blast (24 h apart) exposure, n = 6 per group. * indicates p values < 0.05 as determined by 2-tailed, unpaired, t-tests. Error bars represent standard error of the mean (SEM). (b) Representative western blots* for antibodies used. *For full length blots for each antibody, see Supplementary Fig. S10–S13 online.