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. 2020 Oct 15;10:17426. doi: 10.1038/s41598-020-74171-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Interactions between MdNup54^175–339 and MdKNAT4 as well as MdKNAT6 in Y2H assays. The MdNup54^175–339 truncated sequence was cloned into pGBKT7, whereas MdKNAT4 and MdKNAT6 were cloned into separate pGADT7 vectors. Empty pGADT7 plus MdNup54^175–339-pGBKT7 was used as a control. Additionally, 10⁰, 10⁻¹, 10⁻², and 10⁻³ represent the dilutions of the yeast solution. Yeast cells grown in SD/ − Trp/ − Leu medium and SD/ − Trp/ − Leu/ − His/ − Ade/ + X-α-gal medium are presented.