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. 2020 Oct 15;10:17328. doi: 10.1038/s41598-020-74274-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human blood processing short time-delay has minimal effects on cell viability and RNA yields. (A) Peripheral blood mononuclear cell (PBMC) viable (trypan blue negative) counts after ficoll separation show decreased counts at 15 h 4 °C. (B) Frequency of live singlets in cryopreserved, thawed, stimulated, and stained PBMCs analyzed by CyTOF for unstimulated samples grouped by processing condition. (C) Total RNA yields at all conditions assayed. (D) RNA RIN values at all conditions tested. In all plots, individual outliers were labeled. (n = 10 per condition; *p ≤ 0.05; repeated-measures One-Way ANOVA and BY test for multiple comparisons).