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. 2020 Oct 15;10:17328. doi: 10.1038/s41598-020-74274-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Select cellular features are impacted by processing delay and refrigeration. (A) 26 canonical cell populations were gated and traced between five conditions for unstimulated (left) and stimulated (right) samples. Each condition was compared to baseline for unstimulated or stimulated samples, and (B) significant differences were identified. RT was also compared to 4C for the 5 h conditions. (C) Interindividual variance (CV) for 26 canonical cell populations was calculated for each five conditions for unstimulated (left) and stimulated (right) samples. Statistics: paired two-tailed Student’s T-test with BH method correction for multiple comparisons. Mean fold change was calculated by taking the average of the fold change for each individual. (n = 10 per condition).