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. 2020 Oct 15;11:5196. doi: 10.1038/s41467-020-19042-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Strategy for deriving pericyte-like cells from hiPSC-derived CNCs. b The morphology change during pericyte differentiation from CNCs was detected under phase-contrast microscopy. Scale bar: 100 μm. c qPCR were used for analyze the expression of CNC-specific genes (p75, SOX10, PAX3) and pericyte markers (Caldesmon, NG2, PDGFRβ) during differentiation of pericyte-like cells from CNCs. d. FACS analysis for the surface marker expression of hPSC-CNCs, hPSC-CNC PCs (day 7 and day 14), and HBVPs. Graphs represent the individual data points, the mean ± SEM of three independent experiments. FACS analysis and Bright field images are representative of n = 3 biological replicates. Source data are provided as a Source Data file.