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. 2020 Oct 15;11:5196. doi: 10.1038/s41467-020-19042-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The cell morphology and expression of pericyte markers (Calponin, αSMA, NG2, and PDGFRβ) of hPSC-CNC PCs and HBVPs were revealed by phase-contrast microscopy and immunostaining, respectively. Scale bar: 250 μm (phase-contrast image) and 50 μm (immunostaining image). b qPCR assay for the expression of CNC-specific genes (p75, SOX10, PAX3) in hPSC-CNC PCs and HBVPs. c qPCR assay for the expression of pericyte markers (Vimentin, NG2, Caldesmon, and PDGFRβ) in hPSC-CNC PCs and HBVPs. d The proliferation activity of hPSC-CNC PCs and HBVPs was assessed by CCK8 assay. e A cord formation assay was used to monitor the vascular stabilization potential of hPSC-CNC PCs and HBVPs. Scale bar: 1000 μm. f Total cord length was measured and compared between the hPSC-CNC PC group and the HBVP group. g The retention of total cord length in the hPSC-CNC PC group and the HBVP group was calculated and compared. Graphs represent the individual data points, the mean ± SEM of three independent experiments. Confocal and cord images are representative of n = 3 biological replicates. P-value (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was calculated by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.