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. 2020 Oct 15;11:5196. doi: 10.1038/s41467-020-19042-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a DsRedE2-expressing hPSC-CNC PCs/HBVPs and hrGFP-HBMECs were generated and used for the in vivo Matrigel plug assay. Scale bar: 100 μm. b The localization of DsRedE2-hPSC-CNC PCs and DsRedE2-HBVPs was observed under a fluorescence microscope. Scale bar: 50 μm (left panel) and 10 μm (right panel). c The expression of PDGFRβ in transplanted hPSC-CNC PCs and HBVPs was detected by immunofluorescence assay. Scale bar: 50 μm. d The numbers of blood vessels formed in different groups were counted. e The vessel diameter of different groups was quantified. Graphs represent the individual data points, the mean ± SEM of three independent experiments. Micrographs are representative of n = 3 biological replicates. P-value (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was calculated by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.