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Validation of specific mutants by inoculation onto agar containing different fosfomycin concentrations. Panels (a) and (b) represent analysis of both independent mutants for each gene present in the KEIO collection. For each strain, 5 μL spots representing ∼104 cfu were inoculated and incubated overnight at 37°C. Concentrations are indicated as fractions of the MIC (4 mg/L) for BW25113.
