Table 1.

Loci significantly altered in all exposure conditions

Locus	Predicted impacts of insertiona	Function and interpretation
ahr	Over-expression	Aldehyde reductase
alsR	Inactivation and up-regulation of alsBACEK	Allose binding transporter
cmk	Inactivated	Cytidylate kinase; nucleotide binding
cra	Inactivated	Catabolite repressor/activator. Global regulator of carbon metabolism
crp	Antisense knockdown	Global regulator and carbon catabolite repression
cyaA	Inactivation of regulatory domain	Adenylate cyclase; regulates cyclase activity by carbon source
dacB	Intragenic insert at one site	Peptidoglycan biosynthesis
galU	Inactivated	Synthesis of UDP-d-glucose
glpE	Inactivation and overexpression of glpD	Glycerol metabolism
glpG	Inactivated	Membrane protein protease
glpK	Inactivated	Glycerol kinase; key player in glucose control of glycerol metabolism
glpT	Inactivated	Glycerol uptake; known fosfomycin importer
glpX	Protected	Fructose bisphosphatase
guaA	Inactivation	GMP synthesis
ibaG	Inactivated, inserts upstream of murA	Increase in murA expression
leuA	Protected and over-expression of leuO	Regulation of stringent response, pleiotropic effects from leuO
leuL	Inactivated and over-expression of leuO	Pleiotropic effects from leuO
mlaB-F	Inactivated, inserts upstream of murA	Peptidoglycan recycling; fosfomycin target expression increased
murA	Upstream inserts	Peptidoglycan biosynthesis; fosfomycin target; expression increased
mutL	Protected	DNA repair
nagB	Inactivated with up-regulation of nagE	Over-expression of sugar importer (nagE)
phnC-M	Inactivated (all 14 members of operon in sense)	Phase-variable phosphonate transporter and degradation complex
phoU	Inactivation (downstream of pstB)	Repressor of pstABCS phosphate uptake system
pstABCS	Inactivated	Phosphate uptake system
ptsH	Inactivated	Decoration of imported sugars with phosphates
purA	Inactivated	Purine biosynthesis
rfaH	Inactivated (inserts antisense to tatD)	Transcription anti-terminator
tatD	Protected	DNA repair
treC	Inactivated	Hydrolyses trehalose to glucose
waaFGP	Inactivated	LPS branching
yhfA	Protected and up-regulated	Conserved hypothetical product, known to be Regulated by CRP

^aOver-expression indicates selective advantage for insertions upstream. ‘Protected’ indicates a loss of insertion mutants in the selective condition.