Figure 10.

Cys³²-dependent CAP2 dimer levels and CAP2 association to cofilin are affected in Alzheimer's disease synapses. (A) Representative WB of CAP2 dimer level in the HOMO and TIF hippocampal fraction of APP/PS1 and WT animals. Full uncropped blots are available in the supplementary material. (B) Quantitative analysis shows the decreased ratio of CAP2 dimer OD on the CAP2 monomer OD of APP/PS1 mice compared to WT (TIF, APP/PS1 versus WT, paired t-test: **P = 0.003, n WT = 6 mice, n APP/PS1 = 6 mice). (C) Representative WB showing the levels of CAP2 monomer and dimer in the HOMO and TIF samples of Alzheimer's disease (AD) and HC hippocampal samples. Full uncropped blots are available in the Supplementary material. (D) Quantitative analysis shows that CAP2 dimer/monomer ratio is reduced in AD patients TIF but not in HOMO (TIF, AD versus HC paired t-test: *P = 0.014, n HC = 8, n AD = 8). (E) TIF from hippocampi of HC and AD patients were immunoprecipitated (Ip) with anti-cofilin antibody and CAP2 coprecipitation evaluated in non-denaturating condition. Samples derive from the same experiment and gels/blots were processed in parallel. Full uncropped blots are available in the Supplementary material. (F) Cofilin binding to CAP2 is altered in TIF fraction of AD compared with HC (CAP2 dimer/monomer, AD versus HC, unpaired t-test: *P = 0.023, n HC = 8, n AD = 7).