Table 4.
Pairwise contrasts of interest derived from mixed-effects models introduced in Figs 7 and 8
| Figure | Y | Contrast | Estimate | Standard error | P value |
|---|---|---|---|---|---|
| 7 F | PLA clusters/μm | EGFP-CAP2/Myc-(C32G)CAP2-CTRL versus EGFP-CAP2/Myc-CAP2-CTRL | −0.5914 | 0.1777 | <0.0001 |
| EGFP-CAP2/Myc-(C32G)CAP2-cLTP versus EGFP-CAP2/Myc-CAP2-CTRL | −0.6388 | 0.1777 | 0.00129 | ||
| EGFP-CAP2/Myc-CAP2-cLTP versus EGFP-CAP2/Myc-CAP2-CTRL | 0.5791 | 0.1640 | 0.00129 | ||
| EGFP-CAP2/Myc-(C32G)CAP2-cLTP versus EGFP-CAP2/Myc-(C32G)CAP2-CTRL | −0.0475 | 0.1894 | 0.8030 | ||
| EGFP-CAP2/Myc-CAP2-cLTP versus EGFP-CAP2/Myc-(C32G)CAP2-CTRL | 1.1705 | 0.1777 | <0.0001 | ||
| EGFP-CAP2/Myc-CAP2(C32G)-cLTP versus EGFP-CAP2/Myc-CAP2-cLTP | −1.2180 | 0.1777 | <0.0001 | ||
| 7 H | PLA clusters/μm | Myc-(C32G)CAP2/Cofilin-CTRL versus Myc-CAP2/Cofilin-CTRL | −0.0178 | 0.0104 | 0.115 |
| Myc-(C32G)CAP2/Cofilin-cLTP versus Myc-CAP2/Cofilin-CTRL | −0.0001 | 0.0096 | 0.9901 | ||
| Myc-CAP2/Cofilin-cLTP versus Myc-CAP2/Cofilin -CTRL | 0.0668 | 0.0096 | <0.0001 | ||
| Myc-(C32G)CAP2/Cofilin -cLTP versus Myc-(C32G)CAP2/Cofilin-CTRL | 0.0177 | 0.0102 | 0.115 | ||
| Myc-CAP2/Cofilin-cLTP versus Myc-(C32G)CAP2/Cofilin-CTRL | 0.0846 | 0.0102 | <0.0001 | ||
| Myc-(C32G)CAP2/Cofilin-cLTP versus Myc-CAP2/Cofilin-cLTP | −0.0669 | 0.0094 | <0.0001 | ||
| 8 B | IntDen Cofilin/IntDen RFP | CAP2-shRNA-cLTP versus SCR-CTRL | −0.0051 | 0.0243 | 0.834 |
| SCR-cLTP versus SCR-CTRL | 0.0992 | 0.0218651 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus SCR-CTRL | 0.0245 | 0.0240298 | 0.386 | ||
| shRNA + shr-wt-CAP2-cLTP versus SCR-CTRL | 0.1386 | 0.0239044 | <0.0001 | ||
| CAP2-shRNA-cLTP versus SCR-cLTP | −0.09412 | 0.0236968 | 0.00016 | ||
| CAP2-shRNA-cLTP versus shRNA + shr-C32G-CAP2-cLTP | −0.01937 | 0.0250532 | 0.489 | ||
| CAP2-shRNA-cLTP versus shRNA + shr-wt-CAP2-cLTP | −0.1335 | 0.0238909 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus SCR-cLTP | −0.07475 | 0.0233884 | 0.00246 | ||
| shRNA + shr-wt-CAP2-cLTP versus SCR-cLTP | 0.0394 | 0.0231644 | 0.128 | ||
| shRNA + shr-C32G-CAP2-cLTP versus shRNA + shr-wt-CAP2-cLTP | −0.1142 | 0.0240324 | <0.0001 | ||
| 8 D | Spine length | CAP2-shRNA-cLTP versus SCR-CTRL | 0.3468 | 0.0173 | <0.0001 |
| SCR-cLTP versus SCR-CTRL | 0.2984 | 0.0177 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus SCR-CTRL | 0.3676 | 0.0165 | <0.0001 | ||
| shRNA + shr-wt-CAP2-cLTP versus SCR-CTRL | 0.1881 | 0.0184 | <0.0001 | ||
| CAP2-shRNA-cLTP versus SCR-cLTP | 0.0484 | 0.0180 | 0.00795 | ||
| shRNA + shr-C32G-CAP2-cLTP versus CAP2-shRNA-cLTP | 0.0207 | 0.0171 | 0.2255 | ||
| shRNA + shr-wt-CAP2-cLTP versus CAP2-shRNA-cLTP | −0.1588 | 0.0187 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus SCR-cLTP | 0.0691 | 0.0171 | <0.0001 | ||
| shRNA + shr-wt-CAP2-cLTP versus SCR-cLTP | −0.1104 | 0.0187 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus shRNA + shr-wt-CAP2-cLTP | 0.1795 | 0.0184 | <0.0001 | ||
| Spine width | CAP2-shRNA-cLTP versus SCR-CTRL | 0.0116 | 0.0094 | 0.2185 | |
| SCR-cLTP versus SCR-CTRL | 0.2100 | 0.0096 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus SCR-CTRL | −0.0473 | 0.0090 | <0.0001 | ||
| shRNA + shr-wt-CAP2-cLTP versus SCR-CTRL | 0.2821 | 0.0100 | <0.0001 | ||
| CAP2-shRNA-cLTP versus SCR-cLTP | −0.1984 | 0.0098 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus CAP2-shRNA-cLTP | −0.0589 | 0.0093 | <0.0001 | ||
| shRNA + shr-wt-CAP2-cLTP versus CAP2-shRNA-cLTP | 0.2705 | 0.0102 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus SCR-cLTP | −0.2573 | 0.0093 | <0.0001 | ||
| shRNA + shr-wt-CAP2-cLTP versus SCR-cLTP | 0.0721 | 0.0102 | <0.0001 | ||
| shRNA + shr-C32G-CAP2-cLTP versus shRNA + shr-wt-CAP2-cLTP | −0.3294 | 0.0100 | <0.0001 |
P values were derived after adjustment for multiple comparisons according to the false discovery rate (FDR) approach.