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. 2020 Oct 15;11(10):864. doi: 10.1038/s41419-020-03064-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The protein levels of PRMT5 in four laryngeal carcinoma cell lines and normal oral mucosa epithelial cell were detected by western blotting. b, c Western blotting and qPCR analysis were used to confirm the efficiencies of PRMT5 in overexpression and knockdown of stable cell lines Tu686 and Tu212. d, e Effect of PRMT5 on cell viability was evaluated by CCK8 assay in Tu686 and Tu212 cells. f Effect of PRMT5 on colony formation in Tu686 and Tu212 cells. g Knockdown of PRMT5 increased the G1 fraction, as detected by flow cytometry. All the experiments were repeated three times, and the results are presented as mean ± SD. **p < 0.01.