Table 1.

Kinetic data for binding of ADPr to eAf1521 and WT Af1521, respectively.

Ligand	Ligand density	Model	kon/105 M−1s−1	koff/s−1	KD/nM	χ2	RU (max)
WT Af1521	4650	1:1	2.36 ± 0.02	0.678 ± 0.003	2879 ± 9	0.028	33.3
		ss			9710 ± 1950	0.068	93.2
eAf1521	520	1:1	5.94 ± 0.02	0.001115 ± 0.000002	1.88 ± 0.01	0.042	9.6
eAf1521	500	1:1	5.63 ± 0.06	0.001203 ± 0.000007	2.14 ± 0.01	0.041	10.2
eAf1521	390	1:1	5.15 ± 0.04	0.001000 ± 0.000006	1.94 ± 0.01	0.032	9.5

A dissociation rate constant, k_off, was calculated by using K_D from steady-state measurement and the association rate constant, k_on. Statistical error from fitting.

ss steady state.