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. 2020 Oct 15;10:17483. doi: 10.1038/s41598-020-74564-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Auto-correlation and principal component (PC) analysis of whole transcriptome, DE genes and non-DE genes. (a) Pearson correlation and (b) Gene expression trajectory on PC1-PC2 space between time t₀ (0 min) and t_i (0, 0.5, 1, 2, 5, 10 min for E. coli – left panels) and 0, 30, 60, 120, 180, 240 min for S. cerevisiae – right panel) of whole transcriptome (solid black), DE genes at scatter dot size 0.004 log(TPM) (solid orange), and non-DE genes (dashed pink). The PC trajectories were obtained by taking the average trajectories of 2 replicates. The first 2 PCs account for 91.76% total variance in E. coli and 80.25% in S. cerevisiae.