. 2020 Oct 15;10:17410. doi: 10.1038/s41598-020-74624-6

Table 3.

Inter-observer reliability for the three measurement methods within two groups.

Level	Measurement Method	Observer 1/2*	Observer 3/4†
C1/2	Mean ROI	0.775 (0.595–0.881)	0.510 (0.213–0.721)	0.690 (0.462–0.832)	0.647 (0.400–0.807)
Manual ROI	0.744 (0.546–0.864)	0.635 (0.382–0.799)	0.551 (0.266–0.747)	0.579 (0.304–0.765)
Sagittal ROI	0.809 (0.651–0.900)	0.781 (0.604–0.884)	0.479 (0.173–0.701)	0.430 (0.113–0.668)
C2/3	Mean ROI	0.925 (0.855–0.962)	0.906 (0.819–0.952)	0.652 (0.406–0.810)	0.700 (0.477–0.838)
Manual ROI	0.930 (0.864–0.964)	0.789 (0.618–0.889)	0.747 (0.551–0.865)	0.709 (0.491–0.843)
Sagittal ROI	0.667 (0.428–0.818)	0.814 (0.660–0.903)	0.275 (-0.065–0.558)	0.302 (-0.036–0.578)
C3/4	Mean ROI	0.954 (0.909–0.977)	0.868 (0.752–0.932)	0.682 (0.451–0.827)	0.646 (0.398–0.806)
Manual ROI	0.899 (0.807–0.948)	0.923 (0.851–0.961)	0.792 (0.622–0.890)	0.800 (0.636–0.895)
Sagittal ROI	0.924 (0.853–0.961)	0.907 (0.823–0.953)	0.532 (0.241–0.735)	0.624 (0.366–0.793)
C4/5	Mean ROI	0.948 (0.898–0.974)	0.932 (0.869–0.966)	0.663 (0.423–0.816)	0.757 (0.566–0.871)
Manual ROI	0.967 (0.936–0.984)	0.913 (0.834–0.956)	0.756 (0.565–0.870)	0.773 (0.591–0.880)
Sagittal ROI	0.795 (0.628–0.892)	0.830 (0.689–0.912)	0.422 (0.102–0.662)	0.410 (0.088–0.654)
C5/6	Mean ROI	0.783 (0.608–0.885)	0.580 (0.305–0.765)	0.641 (0.391–0.803)	0.634 (0.380–0.799)
Manual ROI	0.843 (0.708–0.918)	0.838 (0.700–0.916)	0.594 (0.325–0.774)	0.607 (0.342–0.782)
Sagittal ROI	0.676 (0.442–0.824)	0.771 (0.589–0.879)	0.222 (-0.121–0.517)	0.404 (0.081–0.650)
C6/7	Mean ROI	0.596 (0.327–0.776)	0.854 (0.727–0.924)	0.461 (0.150–0.689)	0.555 (0.271–0.750)
Manual ROI	0.728 (0.520–0.854)	0.617 (0.357–0.789)	0.478 (0.1716–0.700)	0.570 (0.291–0.759)
Sagittal ROI	0.649 (0.402–0.808)	0.780 (0.604–0.884)	0.595 (0.326–0.775)	0.628 (0.372–0.795)
C7/T1	Mean ROI	0.892 (0.795–0.945)	0.675 (0.440–0.823)	0.678 (0.445–0.825)	0.694 (0.469–0.834)
Manual ROI	0.861 (0.740–0.928)	0.799 (0.635–0.894)	0.715 (0.501–0.847)	0.679 (0.447–0.826)
Sagittal ROI	0.762 (0.575–0.874)	0.764 (0.578–0.875)	0.084 (-0.257–0.406)	0.157 (-0.186–0.467)

Level

Measurement
Method

Observer 1/2*

Observer 3/4†

1st measurement
ICC(95% CI)

2nd measurement
ICC(95% CI)

1st measurement
ICC(95% CI)

2nd measurement
ICC(95% CI)

C1/2

Mean ROI

0.775

(0.595–0.881)

0.510

(0.213–0.721)

0.690

(0.462–0.832)

0.647

(0.400–0.807)

Manual ROI

0.744

(0.546–0.864)

0.635

(0.382–0.799)

0.551

(0.266–0.747)

0.579

(0.304–0.765)

Sagittal ROI

0.809

(0.651–0.900)

0.781

(0.604–0.884)

0.479

(0.173–0.701)

0.430

(0.113–0.668)

C2/3

Mean ROI

0.925

(0.855–0.962)

0.906

(0.819–0.952)

0.652

(0.406–0.810)

0.700

(0.477–0.838)

Manual ROI

0.930

(0.864–0.964)

0.789

(0.618–0.889)

0.747

(0.551–0.865)

0.709

(0.491–0.843)

Sagittal ROI

0.667

(0.428–0.818)

0.814

(0.660–0.903)

0.275

(-0.065–0.558)

0.302

(-0.036–0.578)

C3/4

Mean ROI

0.954

(0.909–0.977)

0.868

(0.752–0.932)

0.682

(0.451–0.827)

0.646

(0.398–0.806)

Manual ROI

0.899

(0.807–0.948)

0.923

(0.851–0.961)

0.792

(0.622–0.890)

0.800

(0.636–0.895)

Sagittal ROI

0.924

(0.853–0.961)

0.907

(0.823–0.953)

0.532

(0.241–0.735)

0.624

(0.366–0.793)

C4/5

Mean ROI

0.948

(0.898–0.974)

0.932

(0.869–0.966)

0.663

(0.423–0.816)

0.757

(0.566–0.871)

Manual ROI

0.967

(0.936–0.984)

0.913

(0.834–0.956)

0.756

(0.565–0.870)

0.773

(0.591–0.880)

Sagittal ROI

0.795

(0.628–0.892)

0.830

(0.689–0.912)

0.422

(0.102–0.662)

0.410

(0.088–0.654)

C5/6

Mean ROI

0.783

(0.608–0.885)

0.580

(0.305–0.765)

0.641

(0.391–0.803)

0.634

(0.380–0.799)

Manual ROI

0.843

(0.708–0.918)

0.838

(0.700–0.916)

0.594

(0.325–0.774)

0.607

(0.342–0.782)

Sagittal ROI

0.676

(0.442–0.824)

0.771

(0.589–0.879)

0.222

(-0.121–0.517)

0.404

(0.081–0.650)

C6/7

Mean ROI

0.596

(0.327–0.776)

0.854

(0.727–0.924)

0.461

(0.150–0.689)

0.555

(0.271–0.750)

Manual ROI

0.728

(0.520–0.854)

0.617

(0.357–0.789)

0.478

(0.1716–0.700)

0.570

(0.291–0.759)

Sagittal ROI

0.649

(0.402–0.808)

0.780

(0.604–0.884)

0.595

(0.326–0.775)

0.628

(0.372–0.795)

C7/T1

Mean ROI

0.892

(0.795–0.945)

0.675

(0.440–0.823)

0.678

(0.445–0.825)

0.694

(0.469–0.834)

Manual ROI

0.861

(0.740–0.928)

0.799

(0.635–0.894)

0.715

(0.501–0.847)

0.679

(0.447–0.826)

Sagittal ROI

0.762

(0.575–0.874)

0.764

(0.578–0.875)

0.084

(-0.257–0.406)

0.157

(-0.186–0.467)

*The group with two medical students (observers 1 and 2).

^†The group with a radiology resident and neuro-radiologist (observers 3 and 4).

FA, fractional anisotropy, ICC, intraclass correlation coefficient, CI, confidence interval, ROI, region of interest.