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. 2020 Oct 14;10:17187. doi: 10.1038/s41598-020-74275-7

Figure 2.

Figure 2

HMGB1 binds to highly sulfated 12-mers. (a) Illustration of 12-mer structures prepared by chemoenzymatic synthesis. (b) In vitro FXa activity determination for 12-mers with fondaparinux as a positive control. (c) Western analysis of HMGB1 pulldown from liver lysate using biotinylated 12-mers.