Reagent or resource	Source	Identifier
Antibodies
Alpha-smooth muscle actin monoclonal antibody (1A4 (ASM-1))	ThermoFisher Scientific	https://www.thermofisher.com/antibody/product/Alpha-Smooth-Muscle-Actin-Antibody-clone-1A4-asm-1-Monoclonal/MA5-11547
Goat anti-mouse IgG2a cross-adsorbed secondary antibody, AlexaFluor 555	ThermoFisher Scientific	https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG2a-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21137
Pan-cytokeratin monoclonal antibody (AE1/AE3), AlexaFluor 488, EBioscience	ThermoFisher Scientific	https://www.thermofisher.com/antibody/product/Pan-Cytokeratin-Antibody-clone-AE1-AE3-Monoclonal/53-9003-82
Ki-67 (D3B5) monoclonal antibody, AlexaFluor 647, Conjugate #12075	Cell Signaling Technology	https://www.cellsignal.com/products/antibody-conjugates/ki-67-d3b5-rabbit-mab-alexa-fluor-647-conjugate/12075?N=4294960176&Nrpp=100&fromPage=plp
Biological samples
Human PDAC tumor samples	Oregon Pancreas Tissue Registry	IRB: 3609
Chemicals, peptides, and recombinant proteins
SlowFade Gold Antifade Mountant with DAPI	ThermoFisher Scientific	https://www.thermofisher.com/order/catalog/product/S36938
Normal goat serum blocking solution	Vector laboratories	https://vectorlabs.com/normal-goat-serum-blocking-solution.html
Deposited data
Imaging data	This manuscript	https://gitlab.com/eburling/shift/
Software and algorithms
Python	Python Software Foundation	RRID:SCR_008394
PyTorch	Paszke et al.39	https://pytorch.org/
imgaug	Jung40	https://github.com/aleju/imgaug
Netron	https://www.lutzroeder.com/ai/	https://github.com/lutzroeder/netron
HoloViews	Stevens et al.41	https://holoviews.org/
Bokeh	Bokeh Development Team42	https://bokeh.pydata.org/
scikit-image	van der Walt et al.43	https://scikit-image.org/
scikit-learn	Pedregosa et al.44	https://scikit-learn.org
scikit-posthocs	Terpilowski et al.45	https://doi.org/10.21105/joss.01169
SciPy	Virtanen et al.46	https://www.scipy.org/
Jupyter	Kluyver et al.47	https://jupyter.org/