| Hematoxylin | 10 min |
| Wash in water | 1 min |
| Acid alcohol (0.5% HCl in 70% Ethanol) | 8 s |
| Wash in water | 25 s |
| Bluing solution | 2 min |
| Wash in water | 20 s |
| 80% Ethanol/water | 25 s |
| Eosin | 10 s |
| 80% Ethanol/water | 25 s |
| 95% Ethanol/water | 20 s |
| 100% Ethanol (two times) | 25 s |
| Xylene (five times) | 25 s |
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