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. 2020 Oct 14;10:17193. doi: 10.1038/s41598-020-74372-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TDAG8 expression was significantly increased in the cerebral hemisphere after the induction of a transient occlusion for the middle cerebral artery (tMCAO). (a–e) Quantitative analyses of mRNA expression of TDAG8 (a), OGR1 (b), GPR4 (c), Iba1 (d), and GFAP (e) in the contralateral (Contra) and ipsilateral (Ipsi) wild-type (WT) hemispheres after the induction of tMCAO for 0.5 h and reperfusion for 24 h. Total RNA was prepared from each cerebral hemisphere of sham (n = 6) and tMCAO mice (n = 6). Results are expressed as the ratio relative to GAPDH. Error bars represent the mean ± SEM. Comparisons between contralateral and ipsilateral hemispheres were assessed using the paired Student’s t-test (N.S., not significant). The effect of tMCAO and the following reperfusion is significant (p < 0.01).