Figure 4.

The pH declines in the ischemic region as a result of the tMCAO independently of TDAG8 deficiency. (a) Localized pH changes were measured using a pH microsensor in the ipsilateral and contralateral regions of interest (1.5–2 mm lateral, 3–3.5 mm ventral, and 1 mm anterior to the bregma). The predicted position of the pH sensor is indicated by a black arrow on the histogram image of Nissl staining for cell damage 24 h after tMCAO for 0.5 h and reperfusion. (b) Acidification of the ischemic region after MCAO for 0.5 h in WT mice (n = 6) and TDAG8^Tp/Tp mice (n = 8). Error bars represent the mean ± SEM. Comparisons between contralateral and ipsilateral hemispheres were assessed using the paired Student’s t-test. The effect of MCAO was significant (p < 0.01, Contra vs. Ipsi). (c) Restoration of acidic pH in the ischemic region 24 h after the tMCAO/reperfusion in WT mice (n = 3) and TDAG8^Tp/Tp mice (n = 3). Error bars represent mean ± SEM. Comparisons between contralateral and ipsilateral hemispheres were assessed using the paired Student’s t-test (N.S., not significant).