Figure 4.

Representative UV/Vis absorption (turbidity) data at 400 nm of a 50 mg mL⁻¹ solution of γD-crystallin as a function of (a) temperature in buffer (50 mM TRIS, 150 mM NaCl, pH 7.4), 0.3 M urea, 15 wt% Ficoll and 0.3 M TMAO, and (b) pressure at T = 1 °C. Bottom: light microscopy snapshots of γD-crystallin representing the phase-separated and the homogeneous state of the solution. The absorption data are normalized to their maximum values (1.0); the unnormalized data are shown in Figure SI 2).