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. 2020 Oct 2;5(19):e138815. doi: 10.1172/jci.insight.138815

Figure 1. Age-related cortical bone loss requires osteocyte RANKL.

Figure 1

A cohort of female Dmp1-Cre;Tnfsf11fl/fl and Tnfsf11fl/fl littermate controls were aged to 24 months. Another cohort of female mice of the same genotypes euthanized at 8 months of age served as young controls. (A) Sequential BMD measurements in the aging cohort by dual-energy x-ray absorptiometry (n= 8/group). (B) Representative micro-CT images of femoral midshaft cortical bone (scale bar: 100 μm). (C) Cortical bone measurements at the midshaft and (D) cortical porosity at the distal metaphysis of the femur by micro-CT (n = 10–14/group). (E) Cortical thickness and (F) trabecular bone volume (BV/TV) in L4 vertebra by micro-CT (n = 10–14/group). (G) Representative micro-CT images of trabecular bone in L4 (scale bar: 100 μm). Lines and error bars represent mean ± SD. (A) *P < 0.001 versus 5 months of age in the same genotype by repeated-measures ANOVA; ANOVA also showed that BMD was different between genotypes at each age in both the spine and femur (not indicated in graphs). (C–F) P values were determined using 2-way ANOVA.