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. 2020 Oct 2;5(19):e138815. doi: 10.1172/jci.insight.138815

Figure 2. Elevated RANKL and resorption in aged cortical bone.

Figure 2

(A) Representative photomicrographs of nondecalcified femur sections stained for TRAPase activity (red) (scale bar: 100 μm) and quantitation of osteoclast surface per bone surface (Oc.S/BS) measured in longitudinal nondecalcified femur sections of 8- and 24-month-old female Dmp1-Cre;Tnfsf11fl/fl mice and Tnfsf11fl/fl littermate controls (n = 10–14/group). (B) mRNA levels of Tnfsf11 by qPCR of RNA from osteocyte-enriched bone shafts of 8- and 24-month-old female Dmp1-Cre;Tnfsf11fl/fl mice and Tnfsf11fl/fl littermate controls (n = 5–12/group). (C) Tnfsf11 mRNA measured in RNA from osteocyte-enriched bone shafts of 6- and 20-month-old male C57BL/6 mice (n = 9/group). Lines and error bars represent mean ± SD; P values by 2-way ANOVA (A and B) or t test (C).