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. 2020 Oct 2;5(19):e138815. doi: 10.1172/jci.insight.138815

Figure 5. Gata4 overexpression stimulates RANKL production.

Figure 5

Newborn calvaria cells (A–D) or bone marrow–derived stromal cells (E and F) were transduced with retroviruses expressing empty vector or Gata4 and cultured for 24 hours (A–D) or 72 hours (E and F). (A) Protein levels by Western blot. (B and E) Proliferation by BrdU labeling (n = 8/group). (C) Apoptosis by caspase-3 activity (n = 4/group). (D and F) mRNA levels by qRT-PCR (triplicate cultures). (G and H) Bone marrow–derived stromal cells were pretreated with control IgG or neutralizing anti–IL-1a antibody (5 μg/mL) for 30 minutes, followed by (G) recombinant IL-1a (10 ng/mL) or (H) etoposide (10 μM) for 24 hours. mRNA levels by qRT-PCR (triplicate cultures). Line and error bars represent mean ± SD; P values by 2-tailed unpaired t test (B–F) or 2-way ANOVA (G and H).