Figure 1. Characterization of Nna1, Ttll1, and Ttll3.
(A) Nna1 mRNA lacks exons 7–11 in young adult pcdSid cerebrum and cerebellum. (B) Nna1 protein is deficient in young adult pcd cerebellum, hippocampus, and substantia nigra. Dot pots with mean ± SD, n = 4–6; 2-tailed Student’s t test, compared with WT, **P < 0.01. (C) Ttll1 and Ttll3 mRNA were much higher in P20 WT PNs than in WT GNs. The Nna1 mutation reduced Ttll3 mRNA in P20 pcd. Dot pots with mean ± SD, n = 3; 2-tailed Student’s t test, compared with WT, **P < 0.01. (D) Ttll1 and Ttll3 blots were from 2 different gels. Ttll3 protein was decreased in P20 pcd cerebellum. Dot pots with mean ± SD, n = 4–6; 2-tailed Student’s t test, compared with WT, **P < 0.01. (E) Ttll1 protein (green) was equivalent in P20 WT and pcd cerebellum. Calbindin (Calb, red) in PNs, DAPI (blue) in cell nuclei. Scale bar: 50 μm. (F) Ttll3 protein (green) was decreased in P20 pcd PNs, like Calb (red) in the distal area of P20 pcd PN dendritic trees. Scale bar: 30 μm. Nna1, neuronal nuclear protein induced by axotomy; Ttll1, tubulin tyrosine ligase–like 1; pcd, Purkinje cell degeneration; PNs, Purkinje neurons; GNs, granule cell neurons.