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. 2020 Oct 2;5(19):e141264. doi: 10.1172/jci.insight.141264

Figure 1. Characterization of MHC ligands eluted from C18 cartridges with various concentrations of ACN.

Figure 1

(A) Comparison of size-exclusion spin filters and C18 cartridges with 30% ACN elution. (B) Relative changes for the yields of unique HLA ligands between different ACN elution conditions in AML14, JMN, and BV173 cells. (C) GRAVY scores for MHC-assigned peptides in BV173 cells. (D) GRAVY scores of different ACN elution conditions in AML14, JMN, and BV173 cells. (E) Venn diagram for size-exclusion spin filters and C18 cartridge experiments. Data sets were used from the experiment depicted in C. (F) Venn diagram for different ACN elution conditions in AML14, JMN, and BV173 cells. Data sets were used from experiments shown in D. Data were normalized to samples with lowest yield of unique HLA ligand identifications. Key: 30% ACN in red, 40% ACN in green, and 50% ACN in blue. For A and B mean with SD is indicated. In C and D whiskers show range of GRAVY scores from min to max. Boxes show mean with SD. One-way ANOVA test was used for multiple comparisons. ****P < 0.0001.