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. 2020 Oct 2;5(19):e137969. doi: 10.1172/jci.insight.137969

Figure 2. Regulation of glucose metabolism by p53 in fLfs.

Figure 2

(A) hfLfs were transduced with adenoviral vector expressing empty vector (Ad-Ev) or p53 (Ad-p53). Naive hnLfs and hfLfs were used as controls. Forty-eight hours later, the cell lysates were immunoblotted for p53, HK2, PKM, PFKP, PFKFB3, and HIF-1α proteins. Images are representative of 2 independent experiments. (B) Total RNA isolated from n = 4 naive hnLf and hfLfs, or hfLfs transduced with Ad-Ev or Ad-p53 as in A, were analyzed for HK2, PKM, PFKP, PFKFB3, and HIF1A mRNA expression by qPCR (n = 4). (C) hnLfs were transduced with lentiviral vector–expressing control shRNA (Ctrl shRNA) or p53 shRNA. Naive hnLfs and hfLfs were used as controls. After 48 hours, the cell lysates were analyzed for the expression of p53 and glycolytic markers by Western blotting. Images are representative of 2 independent experiments. (D) Total RNA isolated from n = 4 naive hnLfs and hfLfs, or hnLfs transduced with Ctrl shRNA or p53 shRNA, were tested for their mRNA expression by qPCR. Data represented as mean ± SD were analyzed by 1-way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.