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. 2020 Oct 2;5(19):e137969. doi: 10.1172/jci.insight.137969

Figure 4. CSP and CSP7 inhibited augmented glycolysis in fLfs.

Figure 4

(A) hfLfs were treated with or without 10 μM of CSP, CSP7, or CP for 48 hours. The cell lysates were immunoblotted for the differential expression of p53, HK2, PKM, PFKP, PFKFB3, and HIF-1α proteins. The experiments were repeated twice. (B) Total RNA isolated from hnLfs, and hfLfs treated with or without CSP, CSP7, or CP as in A, were analyzed by qPCR for changes in mRNA expression of above listed glycolytic enzymes. Data pooled from 2 independent experiments (n = 4) are represented as mean ± SD. (C) The changes in the cellular lactate contents were measured in untreated hnLfs and hfLfs, as well as hfLfs treated with CSP, CSP7, and CP. Data pooled from 2 independent experiments (n = 4) are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001, by 1-way ANOVA followed by Tukey’s post hoc test.