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. 2020 Oct 2;5(19):e133625. doi: 10.1172/jci.insight.133625

Figure 3. Inhibition of VLO neurons did not affect the anxiodepressive-like behaviors in TN mice.

Figure 3

(A) Schematic of the protocol for the experiments in E–I. (B) Schematic and photomicrograph of coronal section showing AAV-hsyn-hM4Di-mCherry injection into the bilateral VLO. Scale bar: 500 μm for low magnification, 50 μm for high magnification. (C and D) Examples showing that bath CNO (500 nM) increased the injected currents threshold to evoke action potentials (APs) and decreased action potential firing frequencies in AAV-hM4Di-mCherry injection mice (C), but not in AAV-mCherry injection ones (D). (E–I) Inhibition of bilateral VLO neurons failed to change the anxiodepressive-like behaviors of TN mice in EPM and OFT (E–G), FST (H), and TST (I). Two-sided Student’s t test; n = 7 (mCherry) and 8–10 (hM4Di).