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. 2020 Oct 2;5(19):e133625. doi: 10.1172/jci.insight.133625

Figure 8. Effects of D1Rs and D2R agonists into the VLO on TN-induced anxiodepressive-like behaviors and mechanical allodynia.

Figure 8

(A) Schematic of the protocol for experiments in C–G. (B) Schematic and photomicrograph of coronal section showing cannula placement in the bilateral VLO in mice. Scale bar: 500 μm. (C and D) Microinjection of D1R agonist SKF38393 (3 μg, per side) but not D2R agonist quinpirole (1 μg, per side) into the bilateral VLO led to a significant antidepressive effect in FST (C) and TST (D), which can be blocked by D1Rs antagonist SCH23390 (3 μg, per side) in TN mice. *P < 0.05, **P < 0.01, 1-way ANOVA followed by post hoc Student-Newman-Keuls test; n = 8 (NS), 10 (SKF38393), 9 (SCH23390), and 8 (quinpirole; FST, C); n = 7 (NS), 9 (SKF38393), 12 (SCH23390), and 8 (quinpirole; TST, D). (E) Microinjection of D2R agonist quinpirole but not D1R agonist SKF38393 attenuated TN-induced mechanical allodyina. **P < 0.01, 1-way ANOVA followed by post hoc Student-Newman-Keuls test; n = 8 (NS), 10 (SKF38393), and 9 (quinpirole). (F and G) Neither D1R agonist SKF38393 nor D2R agonist quinpirole into the bilateral VLO influenced TN-induced anxiety–like behaviors in OFT (F) and EPM (G). One-way ANOVA; n = 9 (NS), 11 (SKF38393), and 10 (quinpirole).