Table 1. The effects of different mannitol concentrations.

Manitol Concentration(M)	The percentage of protoplast alive in 48 h	The percentage of protoplast alive in 72 h	t
0.4	39.7% ± 4.5% c	18.3% ± 3.5% c	6.46*
0.5	89.0% ± 3.6% a	86.0% ± 5.3% a	0.81
0.6	55.7% ± 5.1% b	50.1% ± 2.2% b	1.78

Notes.

The different lower letters represent the difference at 0.05 level.

^*represents the difference at 0.05 level.