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. 2020 Oct 13;11(41):3712–3722. doi: 10.18632/oncotarget.27759

Figure 3. Oxidative stress, mitochondria, and autophagy in the myocardium of CT26-inoculated BALB/c mice.

Figure 3

(A) Immunostaining of 8-OHdG (oxidative stress). Scale bar, 50 μm. (B) Western blot analysis of LETM1 (mitochondria). (C) Western blot analysis of LC3. LC3-II is a marker for autophagy. (D) Semi-quantification of LETM1 and LC3-II. (E) SDS-soluble myosin light chain-2 (MYL1) measured using ELISA. The signals were standardized with the β-actin signal. Error bar, standard error of data collected from 3 mice. Significant differences were calculated using Student t-test. 8-OHdG, 8-hydroxy-2′-deoxyguanosine; LETM1, leucine zipper and EF-hand-containing transmembrane protein 1; LC3, microtubule-associated protein light chain-3; Control, no tumor control; CT26, mice inoculated with CT26 cells intraperitoneally; ELISA, enzyme-linked immunosorbent assay.