Fig. 2.

Fluorescence microscopy of pancreatic cancer cell lines CFPAC-1 and PANC-1 and pancreatic ductal cell line H6c7 following 4 or 24 h incubation with increasing doses of aminolevulinic acid. CFPAC-1 demonstrated bright ALA-induced fluorescence at all investigated ALA concentrations after 4 or 24 h incubation with ALA. In contrast, PANC-1 showed minimal ALA-induced fluorescence (strongest signal at higher doses of ALA after 24 h exposure). The control cell line H6c7, although showing little fluorescence at lower concentrations, demonstrated increasing fluorescence at higher concentration at both 4 and 24 h. Abbreviations: BF = bright field, FC = fluorescence channel (emission 620 nm), ALA = 5-aminolevulinic acid. 10× magnification, individual image size 877 × 661 μm.