Table 1.
Optimal production conditions for all fusion proteins (FPs)
| Fusion protein | Cell line | Cell density at the time of infection (cells/ml) | rBV stock titer (IFU/ml) | MOI | Culture time (dpi) | Predominant localization of the fusion protein |
|---|---|---|---|---|---|---|
| FP1 | Sf9 | 1.0 × 106 | 3.33 × 108 | 1 | 6 | P |
| FP2 | High Five™ | 1.0 × 106 | 8.27 × 107 | 1 | 6 | P/S |
| FP3 | Sf9 | 1.0 × 106 | 2.13 × 108 | 1 | 6 | P |
| FP4 | Sf9 | 1.0 × 106 | 5.47 × 107 | 1 | 6 | P |
| FP5 | High Five™ | 1.0 × 106 | 5.07 × 107 | 1 | 6 | P/S |
| FP6 | High Five™ | 1.0 × 106 | 4.80 × 107 | 1 | 6 | P/S |
| FP7 | Sf9 | 1.0 × 106 | 1.60 × 108 | 1 | 6 | P |
MOI, multiplicity of infection; P, pellet; S, supernatant