Fig. 8.

Correlation of intracellular Cu content with enzyme activities of TXNRD and GPX. Correlation analysis of intracellular Cu content and enzyme activity of TXNRD (A) and GPX (B) was performed using data of HepG2 cells treated with 100 μM CuSO₄ with or without 50 nM selenite for 72 h. After 48 h of incubation, the two chelators bathocuproine disulfonic acid (BCS, 400 μM) or tetrathiomolybdate (TTM, 75 μM) were added to the cells. The TTM + Cu group was excluded from analysis because of the accumulation of non-bioactive but quantifiable intracellular Cu. *p < 0.05; ***p < 0.001 calculated based on Pearson correlation coefficient.