Fig. 3.

Nox4^−/−mice develop more severe DSS colitis than wildtype mice. Mice were treated for 6d with 2.5% DSS and analyzed on d9. (A) Body weight curve, (B) disease severity score, (C) colon length, (D) histological inflammation, (E) representative colon sections stained with H&E; scale bar 200 μm. Real time PCR data depicting fold upregulation of mRNA in Nox4^−/− mice compared to wildtype mice for (F) Tnfa, (G) Cxcl1 and (H) Ccl5. (A, C, F–H) represented as mean ± SEM, (B, D) represented as median ± IQR. (A) analyzed by one-way ANOVA on day 9, (B, D) analyzed by Mann Whitney test between wildtype and Nox4^−/− DSS groups on day 9, (C) analyzed by one-way ANOVA with Tukey's multiple comparisons test, (F–H) data were log-transformed to a normal distribution then analyzed by ordinary one-way ANOVA with Tukey's multiple comparisons test. Data represent two independent experiments.