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. 2020 Oct 15;11(10):860. doi: 10.1038/s41419-020-03070-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a IHC staining of GATA6 and EMT markers (E-cadherin, N-cadherin and vimentin) in CCA samples. b Correlations between GATA6 and EMT markers in 91 CCA samples by Spearman’s correlation analyses. c Impact of GATA6 knockdown on EMT marker mRNA levels in QBC939 cells by real-time PCR analyses. d Impact of GATA6 knockdown on EMT marker protein levels in QBC939 cells by WB analyses. e Impact of GATA6 overexpression on EMT marker mRNA levels in RBE cells by real-time PCR analyses. f Impact of GATA6 overexpression on EMT marker protein levels in RBE cells by WB analyses. g Impact of GATA6 knockdown on QBC939 cell migration by wound-healing analyses. h Impact of GATA6 knockdown on QBC939 cell invasion by transwell analyses. i Impact of GATA6 overexpression on RBE cell migration by wound-healing analyses. j Impact of GATA6 overexpression on RBE cell invasion by transwell analyses. Control: cells transfected with empty lentivirus vectors. shGATA6: cells transfected with lentivirus vectors encoding short hairpin RNA targeting human GATA6. exGATA6: cells transfected with lentivirus vectors encoding human GATA6 CDS DNA. *P < 0.05.